Impacts of biostimulant (panchagavya), etiolation and media on seed germination and morphological and biological traits of Coconut (Cocos nucifera L.) cv. ALR-1

Abstract :

Axenic culture of the seagrass, Enhalus acoroides (L.f.) Royle was successfully established using seeds which were aseptically dissected out from the fruits. The seedlings were raised in four culture media devoid of growth hormones. Axenicity of the seedlings was analysed by adopting standard marine bacterial / fungal isolation procedures and also by scanning electron microscopy. Of the four media used, medium 1 (10 % filter sterilized coconut water + 1 % charcoal + 15 g agar + filter sterilized UV treated seawater) promoted comparatively good growth of both leaf and root. About 90 % of the seedlings raised in the laboratory in media 1 and 3, (5 % bentonite clay + 15 g agar + filter sterilized UV treated water) grew upto the fourth leaf stage. Hence, medium 1 is recommended for raising axenic cultures of the seagrass, E. acoroides.