GC-MS CHARACTERIZATION OF METHANOLIC EXTRACT OF CARICA PAPAYA LEAVES

Abstract :

Papaya (Carica papaya L.), one of the most studied plant and its belongs to the family Caricaceae. Papaya is commonly known for its nutritional and medicinal value worldwide. Many parts of papaya plant such as roots, leaves, peels, fruits, and seeds have nutritional and therapeutic significance. In ayurvedic medicine, Carica papaya used as medicine from long time. The Present study deals with that papaya leaves have some potential pharmacological activity. Phytochemicals, antioxidants and bioactive compounds of papaya leaves were analysed by Gas Chromatography and Mass spectrometry. Phytochemicals are non-nutrient bioactive components that are primarily responsible for scavenging toxic radicals after oxidative stress by generating antioxidants. In the methanolic leaf extract, triterpenoids, sugars, flavonoids, catechins, saponins and tannins were present. Oxidative stress is a significant player in health and disease and often is known to drive the pathogenesis of several disorders and diseases. The plant extract showed 40- 58% inhibition (in the concentration range of 10-100 μg/mL) against DPPH radical when compared to the ascorbate control (82-93%) inhibition in the concentration range of 10-100 μg/mL. GC-MS analysis of the methanolic extract was performed since it contained diverse classes of phytochemicals. The GC-MS spectrum revealed the presence of 44 compounds. The peaks were integrated and the area under the curve (AUC) was calculated in % to find the overall abundance of the phytochemicals.