Sampling protocol for terrestrial molluscs in the high altitude landscape of the Indian Himalayas

Abstract :

Histochemical localization of leaf and stem of Cleome rutidosperma Wight &Arn. was studied by conventional photonic microscopy. Results of anatomy of leaf and stem showed cellular differentiation. Both the leaf and stem showed secondary growth. The stomata were found to be ranunculaceous type, the cortex and central pith region was occupied by ground parenchymatous cells filled with myrosin cells. Free hand sections of leaf and stem were obtained and treated with respective reagents to localize constituents such as alkaloids, starch, flavonoids, protein, tannin, reducing sugar, phenols and total lipids.