In vitro biological control of Fusarium oxysporum f. sp. cubense (Foc), casual agent of wilt disease in Musa paradisiaca L.

Abstract :

The importance of biofilm study in the environment of the poultry production and processing industries and inhibition of their growth is inevitable to maintain the hygienic atmosphere and ultimately ensure overall quality of poultry. Biofilm forming microbes on the surface of materials can also caused biofouling smell. In the present study, a total of 6 different biofouling bacteria (BFB) were isolated from poultry samples like cage, feeding bottle, tape connections and floor surface of several poultries on nutrient agar medium. Of the 6 different biofouling bacteria, three isolates belonged to the genus Bacillus spp. (BFB1, BFB2 and BFB3), one each belonged to Staphylococcus sp. (BFB4), Micrococcus sp. (BFB5) and Streptococcus sp. (BFB6). The biofilm formation was detected by tube method (TM), Congo red agar (CRA) method and tissue culture plate (TCP) method. The biofilm forming ability of the biofouling bacteria was varied between the species and methods employed. Our study indicates that the tube method is an accurate and reproducible method for detecting biofilm and this technique can serve as a reliable quantitative tool for determining biofilm formation.