Identification of novel inhibitors of Transforming, Growth factor, Receptor by QSAR, pharmacophore modeling, virtual screening and docking analysis

Abstract :

The use of soil rhizosphere bacteria possessing the traits of plant growth promotion under saline stress is becoming prevalent worldwide to achieve sustainable agriculture along with soil reclamation through phytoremediation as well as bioremediation. A detailed study of the natural population in the rhizosphere of three halophytes grown in saline soils was carried out to isolate and identify salt tolerant bacteria that could express plant growth promotion (PGP) traits at high salt concentrations. In the present study, 15 bacteria were isolated from the rhizosphere of halophytes grown in beach soils. Majority of the bacterial isolates were identified as Vibrio sp, Bacillus sp and Pseudomonas sp based on biochemical and morphological observations. The pH and electrical conductivity of the soil were found to be 8 and 6.7 dS m-1 respectively. Out of the 15 bacteria isolated from saline rhizospheres, six isolates showed tolerance to high salt concentration (12.5% NaCl). The potential salt tolerant bacterial isolates were further analyzed for the sodium uptake pattern which showed successive increase in the amount of sodium uptake pattern from 0.1M concentration up to 3M concentration of NaCl. The selected PGPR in this study were adapted to 12.5% NaCl stress and were able to produce IAA, ammonia, HCN, amylase and protease. They also exhibited the ability of phosphate solubilization and nitrogen fixation which would help plants to sequester inorganic phosphate available in the soil and to fix atmospheric nitrogen. The data represented here is quite encouraging to use these PGPR for enhancing plant growth under saline stress conditions.