Phytochemical Constituents and Antimicrobial Effects of Musa Paradisiaca Leaves

Abstract :

The nanotechnology and tools for the synthesis and characterization are developed in various research fields. The microbes were used for the synthesis of nanoparticles in life science studies. Biological approaches were done for the synthesis of TiO2 (Titania) nanoparticles using Fusarium oxysporum. The biosynthesized nanoparticles were employed in the dye decolorization. The synthesized nanoparticle was characterized by techniques like UV-vis spectrophotometer, FTIR and SEM analysis. The process of TiO2 nanoparticles synthesis was optimized for the pH, temperature, metal concentration and time of incubation.

The mycelium of Fusarium oxysporum with 0.025M TiO2 solution was incubated at 27 oC for the synthesis of nanoparticles. The nanoparticles thus obtained were filtered and it was characterized by UV-vis spectrophotometer at 257nm. Further characterization by FTIR and SEM results confirmed the presence of nanoparticles. The optimum conditions for the development of TiO2 nanoparticles were observed at pH 5, temperature 27 oC, metal concentration 10-3 and incubation time period 48 hours. Methyl orange dye was used with different concentrations of synthesized nanoparticles to investigate the decolourization. The samples were collected at various time intervals which showed the efficiency of nanoparticles in the decolourization process due to the broken down of functional group present in the azo dye.