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Scientific Transactions in Environment and Technovation

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Scientific Transactions in Environment and Technovation, | 10.56343/STET.116.012.004.004
Year : 2019 | Volume: 12 | Issue: 4 | Pages : 182-187

Isolation, Characterization and Optimization of Protease using Lactobacillus sp. and Aspergillus flavus isolated from the spoiled fruits

Abstract :

Plant-based mosquitocidal activity has been in use control products generations in traditional practice as a personal protection against mosquitoes bite. Commercial mosquito activities containing plant-based ingredients have gained increasing popularity and are safe when compared to synthetic larvicidal, ovicidal and repellents. The present investigation discovered the larvicidal, ovicidal and repellent activity of methanol extract of E. variegata. Twenty five III instar larvae of Ae. aegypti, An. stephensi and Cx. quinquefasciatus were exposed to various concentrations (50-250 ppm) in the laboratory by using the standard protocol described by WHO (2005). The ovicidal activity was determined against Ae. aegypti, An. stephensi and Cx. quinquefasciatus mosquito to various concentrations ranging from 70-350 ppm under the laboratory conditions. The repellent activity of E. variegata chemical compositions tested at concentrations of 5.0 mg/cm2 and evaluated in a net cage (45×45× 40cm) containing 100 blood starved female mosquitoes of Ae. aegypti, An. stephensi and Cx. quinquefasciatus using the protocol of WHO (1996). LC50 and LC90 values of the methanol extract of E. varegata were found to be 121.70, 129.29, 134.64 and 217.71, 232.55, 267.40 mg/L against Ae. aegypti, An. stephensi and Cx. quinquefasciatus, respectively. Ovicidal activity of methanol extract was assessed by assessing the egg hatchings. Highest concentrations 240 and 300 ppm of the extract exhibited 100% ovicidal activity against Ae. aegypti, An. stephensi and Cx. quinquefasciatus. The repellent activity of methanol extract E. variegata was found to be the best and therefore the activity was ascertained at 5.0 mg/cm2 concentration provided 100% protection up to 90, 120 and 150 min against Ae. aegypti, An. stephensi and Cx. quinquefasciatus. The compounds present in the leaves of E. variegata were analysed and determined by using GC-MS . The results clearly showed the presence of active compounds in leaves of E. variegate, and the bioactive compounds would pave way for the discovery of new drugs.

Keywords:

Erythrina variegata, Aedes aegypti, Anopheles stephensi, Culex quinquefasciatus, larvicidal, ovicidal and repellent activity.

Citation: *,

( 2019), Isolation, Characterization and Optimization of Protease using Lactobacillus sp. and Aspergillus flavus isolated from the spoiled fruits. Scientific Transactions in Environment and Technovation, 12(4): 182-187

Mr.Veerapathiran K

Correspondence: M.Kannahi


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