Effect of Malus domestica(Apple) fruit puree and Lycopersicon escultentum Mill (tomoto) puree as fat replacers on the sensory properties of a plain shortened cake

Abstract :

An efficient protocol for in vitro propagation of phyllanthus niruri L. has been develpoed. MS medium supplemented with NAA induced prolific callus from leaf, shoot tip and nodal explants. Organogenic and chlorophyllous calli produced well developed prominent shoots with thick and long roots in the medium supplemented with NAA ( 1 mg/I) and BAP ( 1 mg/I). Of the in vitro grown 120 plantlets transferred to the field, 92% survived after two months of transplantation to natural environment.