Numerical solution of magneto-hydrodynamic and hematocrit on blood flow

Abstract :

Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in Gram-negative isolates. ESBLs are mainly coded for b three important genes, namely blaTEM, blaSHV and blaCTX-M. Multiplex PCR as a rapid method has been used to identify two common genes (blaCTX-M and blaSHV) responsible for extended spectrum beta lactamase production in members of Pseudomonadaceae family isolated from different clinical samples from a speciality hospital at Chennai. A total of 10 non repetitive clinical isolates of 24 patients from urine was selected for study. Phenotypic identification for ESBL production was confirmed by multiplex PCR for blaCTX-M and blaSHV performed for the ESBL positive isolates. Among the ESBL strains of P.aeruginosa, 8 isolates were found to be positive for blaSHV gene and á isolates were found to be positive for blaCTX-M3 like gene. The present study demonstrates rapid detection of blaSHV and blaCTX-M in isolates belonged to Pseudomonadaceae using multiplex PCR. This genotypic method provided rapid and efficient differentiation of ESBLs in the laboratory.