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STET Journals.,   Volume(11) - Issue(4), 2018
pp 218-221,   DOI:

Gas Chromatography - Mass Spectrometry (GC-MS) analysis of bioactive compounds present in methanolic extract of the flowers of Punica granatum Linn.



The chemical components were analysed from the methanolic flowers extract of Punica granatum by using Perkin Elmer Gas chromatography and Mass spectroscopy (GC-MS). The results of GC-MS compounds in the extract were relevant to the National Institute of Standards and Technology (NIST) library. GC-MS analysis of the methanolic extract of Punica granatum flowers revealed the presence of 24 compounds including isoquerecitrin, 1,2,3 benzenetriol, N-hexadecanoicacid, 5,6,7,8,22 ,52 - hexahydroxy-42 -methoxy flavanone-7-â-D xylopyra noside (granatumflavanylxyloside), punicanyl benzoate, uroslic acid, 3,7,8,4’-tetrahydroxy-3’-myrt-8-enylflavone, squalene, and masilinic acid. The highest peak area (%) of 48.16 was obtained by 3,7,8,4’-tetrahydroxy-3’-myrt-8-enylflavone with retention-time 10.90 and the lowest peak area of 0.76% was obtained in Beta sitosterollaurate with the retention time of 34.98. Thus this type of GC-MS analysis is the first step towards understanding the nature of active principles present in this medicinal plant. The phytochemical analysis proved that Punica granatum could be a new potential source of raw materials for the pharmaceutical industry.


Gas chromatography; Mass spectrum; Punica Granatum; Methanolic extract

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