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Research Article

Year : 2018 | Volume: 10 | Issue: 3 | Pages: 105-108

Elicitation of antioxidants and phenolics in Vigna radiata L. Wilczek by twin PGP bacteria against infection of Macrophomina phaseolina Tassi (Goid.)

Chankee Kumar Sharma1*, R.C. Dubey2

DOI: 10.20894/STET.116.010.003.001

Corresponding author

Chankee Kumar Sharma*

Department of Botany and Microbiology, Gurukul Kangri University, Haridwar - 249404, India.

  • 1. Department of Botany and Microbiology, Gurukul Kangri University, Haridwar - 249404, India.
  • 2. Department of Botany and Microbiology, Gurukul Kangri University, Haridwar - 249404, India.

Received on: 01/11/2016

Revised on: 02/01/2017

Accepted on: 02/01/2017

Published on: 15/03/2017

  • Chankee Kumar Sharma, R.C. Dubey (2018), Elicitation of antioxidants and phenolics in Vigna radiata L. Wilczek by twin PGP bacteria against infection of Macrophomina phaseolina Tassi (Goid.). Scientific Transactions in Environment and Technovation, 10(3): 105-108. doi: 110.20894/STET.116.010.003.001

Abstract

Plant growth promoting Pseudomonas putida CRN-09 and Bacillus subtilisCRN-16 screened from the rhizosphere of Vigna radiata were used to determine antioxidant activity and total phenolic content in germinated seedlings. Seeds of V. radiata Pant M5 bacterized with P. putida CRN-09 and B. subtilis CRN-16 individually as well as consortium showed better effects on V. radiata seedlings. Maximum antioxidant level (4.20 mM/g) was determined in consortium-treated seedlings at day 6 of treatment. Consortium of both the isolates also enhanced the maximum level of total phenolic contents (1.20 μg/g) at 6th day. Antagonistic effect of seedling extracts of different treatments was evaluated against M. phaseolina. Maximum inhibition area (5 mm) around extract of consortium on first day and 12 mm at 5thday were recorded.

Keywords

Pseudomonas putida CRN-09, Bacillus subtilis CRN-16, Macrophomina phaseolina, antioxidant activity, total phenolic content.