Effect of endosulfan on the bacterial and fungal populations in the gut of
the Indian earthworm Lampito mauritii (Kinberg)

Abstract :

High sensitive C-reactive protein (hsCRP) from the Lipemic serum hsCRP was isolated by using calcium dependent affinity chromatography of CNBr activated Sepharose – 4B covalently coupled to p- diazonium phenyl phosphoryl choline (DPPC)and beads coated with anti hsCRP. The molecular weight of hsCRP was determined using SDS- PAGE. The purity was also determined using radial immunodiffusion technique. The elute obtained from affinity column was evaluated using nephlometer to know the hsCRP levels in serum. The differences in the concentration of elute of affinity chromatography was also studied. The whole study was done using serum samples having very high cholesterol, triglyceride and LDL cholesterol values. The purified hsCRP was homogeneous as determined by using immunodiffusion technique. The molecular weight of hsCRP was approximately 23.6 KD - SDS polyacrylamide gel electrophoresis. The results of single radial immunodiffusion technique showed a better and a larger ,clear ring both in the case of males and females than the well with direct serum. The present investigation deals with study of relationship between hsCRP and high amount of lipids in the serum .and also involves the analysis of hsCRP in predicting early cardiovascular problems. Early prediction of cardiovascular problems can help in reducing the mortality rate due to cardiac diseases which is now becoming the reason for the relatively high rate of deaths across the world.