Evaluation of in vitro anticancer potential of Achyranthes aspera against EAC cell line
- 1, ,
Cancer is a disease in which there is an uncontrolled multiplication and spread, within the body, of abnormal forms of
the body’s own cells. Assessment of in-vitro cytotoxicity has been recently become popular as a primary screening
method for evaluating anticancer activities of various natural sources. This study was carried out to characterize anticancer activity of aqueous and ethanolic extracts of the whole plant of Achyranthes aspera L on Ehrlich Ascites carcinoma cell line [EAC] by MTT [(3- (4,5 dimethyl thiazol-2-yl) 2,5-di phenyl tetrazolium bromide] assay. Aqueous and ethanolic extracts of the whole plant of A. aspera have a significant anticancer effect against EAC cell line in the concentration range between 15.62ìg and 250ìg determined by using MTT assay. The minimum inhibition of aqueous extract showed 6.11% at 15.62ìg/ml and maximum inhibition 59.07% was observed at 250ìg/ml. IC50 value of A. aspera on EAC cell line was 192.64ìg/ml by MTT assay. The minimum inhibition of ethanolic extract showed 7.80% at 15.62ìg/ml and maximum inhibition 68.77% was observed at 250ìg/ml. IC50 value of A. aspera on EAC cell line was 156.51ìg/ml by MTT assay. It is concluded that the extract of A. aspera has potent anti-cancer activity on EAC cell line.
Keywords: Anti-cancer, MTT Assay, EAC Cell Line, Achyranthes aspera.
Citation: *, ( 2017), Evaluation of in vitro anticancer potential of Achyranthes aspera against EAC cell line. Scientific Transactions in Environment and Technovation Journal(STET), 10(3): 122-125
Received: 2015-03-05 10:42:43; Accepted: 03/01/2017;
*Correspondence: unknow user, unknow user