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Research Article

Year : 2007 | Volume: 1 | Issue: 1 | Pages: 15-18

In vitro propagation of Withania somnifera (L.) Dunal from shoot tip and nodal explants.

Karthikeyan1*, 2

DOI: 0

Corresponding author

Karthikeyan*

Anna Unversity

  • 1. Anna Unversity

Received on: 17/06/2015

Revised on: 17/06/2015

Accepted on: 17/06/2015

Published on: 17/06/2015

  • Karthikeyan, (2007), In vitro propagation of Withania somnifera (L.) Dunal from shoot tip and nodal explants.. Scientific Transactions in Environment and Technovation, 1(1): 15-18. doi: 10

Abstract

Withania somnifera L. called as "Ashwagantha" in India, is an important medicinal plant equivalent to ginseng. It is used as stimulant, aphrodisiac,diuretic and tonic. For mass multiplication, shoot tip and nodal explants were inoculated in the Murashige and Skoog’s medium fortified with various growth regulators such as 6-benzyl arninopurine, indole-3-butric acid, naphlllalene acetic acid, kinetin and gibberellic acid, Nodal explants responded better than the shoot lip explanls and gave maximum shoots on BAP + Kn+ NAA (0.5 to 2.0 mg/l) Supplemented medium. Shouts transferred into the rooting medium containing NAA 0.5 mg/l and IBA 1 mg/l gave good result (80%). The rooted plantlets were hardened under green house and transferred to the field.

Keywords

explants, in vitro propagation, Withania and samnifera.